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In modern times, likely caused by increased selection pressure due to the extensive use of antibiotics, mobile genetic elements including conjugative plasmids, integrons and transposons tend to be clustered together with ARGs forming mobile multidrug-resistant units.
Elevated conservation in ubiquitous PKs likely reflects increased selection pressure on housekeeping kinases.
Suppression of reinfection provides longer disease free intervals but at the expense of increased selection pressure for resistance to piperaquine compared with more rapidly eliminated partner drugs.
Even though the levels found in the environment are low, in general below the minimum inhibitory concentration for most bacteria, they may still result in an increased selection pressure at some sites [4], [6].
Finally, as also shown above, an increased selection pressure makes the cyclic dynamics more robust to higher levels of induced switching.
A restrictive antibiotic subscription policy is needed to avoid an increased selection pressure where integrons play a potential significant role for the uptake and dissemination of resistance genes.
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When faced with new stressful conditions and increased selection pressures, organisms can respond in several ways.
Taken together, our results support both developmental constraint acting to limit the divergence of early expressed, developmentally important genes [ 5, 8], as well as the notion that accelerated divergence in adults is primarily due to increased selection pressures occurring during this stage.
For instance, increasing N favours the exploration of the landscape, whereas increasing selection pressure directs the search towards the exploitation of the most promising solutions.
Evolutionary engineering is a useful tool to obtain a desired phenotype by acquiring a stable genotype in an organism by applying constant or increasing selection pressure (Almario et al. 2013; Sauer 2001).
The phage that are thus selected are subsequently amplified in E. coli and undergo additional rounds of panning, often at progressively increasing selection pressure (e.g., by using decreasing concentrations of ligand for later rounds of panning) to enrich the strongest ligand-binding protein fragments.
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