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On the other hand, genetic disruption of PARG also leads to increased level of cell death by accumulation of PAR.
Asterisk denotes significantly increased level of cell viability compared with trypan blue measurement (p < 0.05).
Correspondingly, flow cytometry analysis also revealed increased level of cell surface density of TLR2 and TLR4 in B. pseudomallei- infected cells at 24 h post infection (Figure 2B).
This result was also confirmed by increased level of cell caspase-3 activity (Fig. 4b).
This reduced amount of cell death and increased level of cell proliferation possibly resulted in hypertrophy of the URS epithelia at E11.5.
Flow-cytometry analysis confirmed an increased level of cell-surface expression of uPAR in the MDA-MB-231Hi cells relative to MDA-MB-231 cells.
We found an increased level of cell surface EGFR in DiFi cells following the treatment with chronic doses of the anti-EGFR mAb ICR62.
Cells treated with 5 mM ATP showed a significantly increased level of cell death (19.03±0.67%) compared with untreated cultures (11.59±0.59%, **** P<0.0001, Figure 6c).
This observation strongly suggests that increased level of cell surface EGFR in DiFi62 cells may be caused, in part, by dysregulated receptor internalisation and/or degradation as a result of alterations in the tyrosine kinase domain of the EGFR.
It is not known whether the cell death observed with the Cx31 EKV mutants in vitro also occurs in vivo, and, if so, how this increased level of cell death relates to the disease pathogenesis of EKV.
The significance of increased PBMC apoptosis in SLE is profound, possibly reflecting increased levels of cells undergoing activated induced cell death and/or a defect in non-inflammatory phagocytosis of apoptotic cells.
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