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The fluorescence intensity of native 6-coumarin decreased with increased incubation time, i.e. 2, 24, 48 and 120 h.
The increased incubation time leads to a fourfold increase in detection signal in the reaction chamber with the longer incubation time.
Moderate increase in density of the minerals on PPEES 2 composite after 5, 15, and 30 days of incubation in SBF solution was evident (Figure 3b e) due to the presence of nHA, and it clearly elucidated that the increased incubation time improved the enhanced apatite formation.
Data showing that a clusterin knockout mouse inoculated with BSE prions has a significantly increased incubation time suggested that Clu was a particularly good candidate quantitative trait gene for prion disease incubation time [25].
GFP is known to show non-equilibrium behavior in denaturant-induced unfolding [27] (consistent with the unfolding transitions shifting towards lower Gdn-HCl concentrations at increased incubation time (cf. Fig. S6B and C)), so true free energies of unfolding cannot be deduced from unfolding transitions alone.
According to the previous studies [23], [24], the following conditions were recommended in order to decrease recombination frequencies during long RT-PCR: the minimum quantity of input HIV-1 RNA, use of mixtures of DNA polymerase and proofreading enzymes, increased incubation time for cDNA synthesis, and increased extension time and a minimum number of cycles for PCR.
Similar(41)
After 10 min, the reduction was about 10 thousandfold with the same cod trypsin concentration, and further reduction was seen with increased incubation times.
With increased incubation times the measured increase in cell yield and aggregate size was a result of a much greater portion of the cell monolayer becoming detached from the culture surface.
However, similar intervention at 24 h and 2 days after low-dose infection produced substantially increased incubation times in one out of 5 animals per group, each (table 1).
The main modifications of extraction protocols from wood in comparison to fresh tissue refer to careful mechanical disruption of wood, use of protective chemicals, and often considerably increased incubation times.
PV increased with increasing incubation time and temperature.
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