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Specifically, expression levels of genes encoding putative virulence factors (e.g. LAC1, LAC2, CAS3 and MPK1) and genes encoding proteins involved in cell wall assembly, carbohydrate and lipid metabolism were increased in strain R265, whereas genes involved in the regulation of mitosis and ergosterol biosynthesis were suppressed.
The number of unaffected cells (after zymolyase treatment) was markedly increased in strain tSEC6 with DOX compared to controls (Fig. 5D).
As expected, the levels of these compounds were relatively low for the NRRL1951 strain, increased in strain Wisconsin 54-1255, andramaticallyly increased in DS17690, in particular during the late growth phase.
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However, even though the efficiency of homologous integration events is tremendously increased in strains deficient in NHEJ, it remains highly locus-dependent (Schuster et al. 2012).
In addition, this novel species forms biofilms on the hydrophobic surfaces of polystyrene, and motility is increased in strains displaying smooth colony morphology.
The accumulation of single-stranded regions is increased in strains deficient in translesion synthesis, homologous recombination, or the DNA damage checkpoint (Lopes et al. 2006).
In media lacking Met and Cys, the expression of GFP was greatly increased in strains carrying the pCU-MET3-GFP plasmids.
Most interestingly, when oleic acid serves as the carbon source the ratio of triacylglycerol accumulation in mutants to wild-type is significantly increased in strains defective in DGA1 but not in lro1Δ.
Indeed, KGDH activity was significantly increased in ΔpkaA strain than when compared to the wild-type strain (Fig. 7e).
3-NT gradually increased in all strains during HBO(2) but accumulation was slower in nNOS mice, consistent with less ONOO production.
Surprisingly, the peak of Cyc8p was significantly increased in tup1 strains.
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