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In contrast to one-photon microscopy, two-photon probe-based fluorescence imaging can provide improved three-dimensional spatial localization and increased imaging depth.
Of more serious consequence, is the loss of sectioning with increased imaging depth.
We repeated the experiment with an increased imaging depth by inserting 8-10 mm of chicken breast between the rats (n = 5) and the imaging pad.
It is noted that simultaneous multiphoton excitation has been widely applied in fluorescent optical microscopy to show increased resolution, decreased specimen autofluorescence, as well as increased imaging depth.
Since SD-OCT has the highest sensitivity near to zero-delay and sensitivity decreases for larger delays, by inverting the OCT image, the choroid is closer to the zero-delay line, providing enhanced sensitivity and increased imaging depth.
It has the highest sensitivity near to zero-delay and that sensitivity decreases for larger delays; an inversion of the OCT image was proposed, to move the zero-delay line closer to the choroid, providing enhanced sensitivity and increased imaging depth.
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Multiphoton microscopy has several advantages over confocal and traditional fluorescence microscopy, including decreased out of focus photodamage, increased imaging depths and intrinsic optical sectioning [11].
A more systematical study in Figs. 5(c) and 5 d) shows how the image contrast diminishes with the increasing imaging depth for both regular 2P imaging and SERF.
As smaller fringe periods (i.e. higher fringe frequencies) correspond to deeper imaging depths, this reduced visibility results in decreasing sensitivity with increasing imaging depth.
However, combining this mechanism with a spectral domain CPOCT system by moving the imaging tip solved the problem of degraded lateral resolution with increasing imaging depth.
This value is a best-case scenario, since the Strehl ratio of the beam decreases with increasing imaging depth in tissue.
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