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Also increased imaging time results in increased count number and better image quality.
Immunostained thick femoral slices were successively washed in PBS overnight and incubated in RapiClear 1.52, for a minimum of 6 h, which typically increased imaging depth to 150 µm from the tissue surface without significant loss of signal intensity.
In contrast to one-photon microscopy, two-photon probe-based fluorescence imaging can provide improved three-dimensional spatial localization and increased imaging depth.
Multiphoton microscopy has several advantages over confocal and traditional fluorescence microscopy, including decreased out of focus photodamage, increased imaging depths and intrinsic optical sectioning [11].
The introduction of MSCT with four, eight and 16-detector systems has significantly increased imaging speed.
This enables increased imaging speed, and a corresponding reduction in sample light exposure, when imaging a plane, or planes, within an extended volume.
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It is shown how the use of seven channels increases imaging field of view and improves signal-to-noise ratio for the hand images.
The main limiting parameter of increasing imaging speed in tapping mode is the time constant [τ c ] of the cantilever.
To increase imaging speed, the EPI pulse sequence incorporates temporal (m) and spatial multiplexing (n) with an increased number of image slices (mxn) acquired in a single EPI echo train, thus Multiplexed-EPI (M-EPI).
However, with increasing imaging speed, the photon output of fluorophores also has to be improved.
While NBI-guided TUR has been reported to increase imaging quality versus unguided TUR, neither a meta-analysis nor a RCT comparing the two techniques has been performed.
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