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Hence, the maximal FRET signal in the translocation assay reflects the actual amount of (active) transporters in the system, thus explaining the increased FRET signal for membranes with increased levels of FtsW and decreased FRET signal for membranes with depleted levels of FtsW.
The observation is consistent with the increased FRET from the 1,8-naphthalimide (donor) to the ring-opened, colored form of rhodamine (acceptor).
The resulting constructs once more showed increased FRET efficiencies (E = 0.28 and 0.31, respectively), although they did not surpass the CFPΔ-linker-Venus construct (E = 0.31).
Increased FRET signal between the bound and exchanging peptide in the presence of DM provides support for the requirement of a tetramolecular intermediate in the mechanism of DM-mediated exchange.
The results show that GSK101 does not lead to an increased FRET transfer efficiency between the TRPV4 subunits, or more specifically at the tagged C-termini, on the plasma membrane, indicating that the GSK101-induced TRPV4 activation does not involve theassembly of TRPV4 subunits into new homotetrameric structures.
Moreover, increased FRET was also well correlated with Smo activation.
Similar(46)
It increased fretting fatigue strength (FFS at 107 cycles) of the Ti6Al4V alloy by approximately 10%.
The most important design and patient factors predicting increased fretting and corrosion scores of the ceramic head cohort in this study were stem material, flexural rigidity, and body weight.
The obtained results show that a high degree of cold expansion does not increase fretting fatigue life.
This retrieval study documents taper damage at modular interfaces in retrieved MOM THA systems and investigates if increased modularity is associated with increased fretting and corrosion.
Whilst protecting against dislocations [ 39, 40], maximizing the head-neck ratio with the use of shorter, smaller tapers may also result in increased fretting and corrosion [ 2].
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