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To represent genes with a high constitutive expression value in TCO and a slightly increased expression value in SFED, the beta-1,4-glucanase gene CEL1 (BGlu1) was used (Tables 1 and 3, Figure 5).
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Finally, a sesquiterpene synthase (TPS) showed very low constitutive expression values in SCO but had high expression values in both TCO and TFED and slightly increased expression values in SFED (Table 3, Figure 5).
From this group 85 genes had increased expression values (Additional file 1) and 99 genes showed decreased expression values (Additional file 2), therefore both files represent candidate genes of interest in adipogenic development.
In accordance with the increased apoptosis initiator caspase-8 activity determined in traumatic IVD cells, the analyses of the cell death receptor FasR mRNA revealed significantly increased expression values when compared to non-traumatic controls (p < 0.05).
In principle, these mRNAs could still be authentic targets that are repressed in these cells but nonetheless had increased expression values because either experimental noise or secondary effects of introducing the miRNA overwhelmed the signal for miRNA-mediated repression.
Cell types are ordered by increasing expression values from left to right.
We defined 'alpha outliers' as having an α i,j in the top 10%% of all values, and calculated the outlier rate for bins of increasing expression values.
In addition, eight hemicellulase genes showed increased expression with an average RPKM value of 344, and five hemicellulase genes responding to carbon starvation had an average expression value of 52.
The the value of 2−ΔΔCt>1 reflects increased expression of the target PCMT gene, and a value of 2−ΔΔCt<1 points to a decrease in the gene expression [45].
In contrast, collagenase injected rats showed a significantly increased expression of TRPV1, with a value of 37.1 ± 2.2% positively labelled neurons (P <0.01, Figure 8C).
148 significant genes with negative R values showed reduced expression, and 256 with positive R values showed increased expression, as maze performance worsened (Fig. 5A).
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