injection of neutralizing anti-mouse IgM antibody resulted in significantly increased clinical scoring and reduced body temperature indicating a more severe pulmonary infection 9 hours after induction of pulmonary infection.
Stride frequency (the average number of times a paw contacts the belt per second) increased linearly with increasing clinical score.
The scoring system was then applied to the validation group showing that the incidence of GDM increased with increasing clinical score values ranging from 0.9 in women who scored ≤1 to 18.7% in women who scored ≥6.
This area progressively increased in animals with increasing clinical scores.
The stance time (the weight-bearing portion of the stride in which the paw remains in contact with the belt) decreased progressively with increasing clinical scores.
Consistent with a decrease in stride time, the time spent in these four phases also decreased linearly with increasing clinical scores.
Using DigiGait, we are able to quantify the maximal paw area in contact with the treadmill during the stance phase of the step cycle and demonstrate that the paw area increased with increasing clinical scores.
In the present study, we have observed that the stride frequency indeed increased and the stride length decreased linearly with increasing clinical scores, thus confirming this important observation in the current CIA model.
Subsequently, with an increase in stride frequency at a fixed walking speed (15 cm/s), stride length (the distance between initial contact of the same paw in a complete stride) and stride time progressively decreased with increasing clinical scores.
The propulsion time (the period between the stance phases and beginning of the swing phase when the paw is not in contact with the belt) decreased progressively with the increasing clinical scores.
