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Rollin et al. showed that treating switchgrass with organozolv resulted in a similar increase in the surface area causing increased cellulase adsorption [ 88].
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Increasing cellulase adsorption and cellulose digestibility by decreasing particle size has previously been reported [ 14- 16].
The results indicated that P. chrysosporium increased the total lignin content in residual biomass and also increased the cellulase adsorption kinetics in the resulting lignin.
The pretreatment with swollenin caused a significant decrease in particle size as well as in crystallinity of the cellulosic substrates, thereby substantially increasing maximum cellulase adsorption.
The pretreatment with swollenin resulted in a significant decrease in particle size of the cellulosic substrates as well as in their crystallinity, thereby substantially increasing maximum cellulase adsorption onto these substrates.
Since no further increase in cellulase adsorption was detected after 1.5 h (data not shown), adsorption equilibrium was verified.
The relative increase in cellulase adsorption between the pretreatment with swollenin and the pretreatment with buffer (filter paper > α-cellulose > Avicel > Sigmacell) showed a similar series as the relative reduction in mean particle size (filter paper > Avicel > α-cellulose > Sigmacell; Figure 5).
However, the strong reduction in the CrI of α-cellulose explains the disproportionate increase in maximum cellulase adsorption onto α-cellulose (Table 1), since cellulase adsorption can increase with decreasing CrI [ 26].
However, in the case of α-cellulose, the increase in maximum cellulase adsorption was disproportionately higher.
However, the pretreatment with swollenin caused a significant increase in maximum cellulase adsorption except for Sigmacell.
This may be explained by the substantial decrease in mean particle size and the strong increase in maximum cellulase adsorption (Table 1) for filter paper by swollenin pretreatment.
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