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Increased binding caused delay in the apparent fluorescence kinetics (Fig. 3b).
Indeed, CSA 8M showed greatly increased binding to TCP1 when compared to CSA WT (Fig. 5c).
The increased binding of the M535I mutant to PHD2 was not observed in this experiment.
Expanded CAG repeats in the huntingtin protein show increased binding to HAP-1.
We found that CIP treated ULK1 increased binding, but kinase treated ULK1 decreased the binding (Supplementary Figure 5c).
We also observed increased binding of STX17 to SNAP29 in ULK1 WT and S423A transfection with CQ treatment (Fig. 5e).
For the transcription factor TFIIB, autoacetylation at K238 allows for increased binding to TFIIF and strong activation of transcription28.
The addition of unpaired single strands to blunt DNA ends increased binding and activation of the kinase.
The change in molecular configuration of hemoglobin that accompanies the release of oxygen leads to increased binding of carbon dioxide to oxylabile amino groups.
This might be also due to the altered cytoskeleton which might promote increased binding of free APOA1 to the macrophage surface.
It means GR most likely can not be blocked by MBPs in the promoter region, due to its increased binding affinity to methylated sites.
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