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Increased amplification success with lower template amounts may also be seen.
QMSP using LNA probe and primers showed an increased amplification efficiency and maximum fluorescent signal.
Increased amplification of DNA templates was observed following WGA; however, no amplification advantage for ssDNA ligase treatment of templates was evident compared to linear templates.
The addition of a dilution step and a concentration step before applying the Qiagen protocol further increased amplification of β-globin (from 50 to 63%) and the HPV L1 gene (from 13 to 33%).
In this study we proposed to exploit this increased amplification power for use with minimal input RNA amounts extracted from any tissue matrix.
Further opportunity for increased amplification power when using RNA input within the picogram range is offered by the One-Direct RNA amplification system, designed for use with high quality RNA ranging from 500 pg down to 10 pg of input.
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Thus, incorporating LNAs into PCR primers can increase amplification success, sensitivity, and performance under a wide range of conditions.
He said that with increasing amplification of the issue on social media, he worried that Muslims would be blamed for what some would wrongly perceive as a canceling of Christmas.
In previous designs, most of the light produced by the laser leaked out, which required researchers to increase amplification of the remaining light energy to sustain the laser operation.
Although a nonlinear hearing instrument can compensate by increasing amplification for soft sounds, it cannot compensate for the loss of suprathreshold abilities such as impaired frequency resolution.
Because energy is conserved, the RF field will increase (amplification) if the electrons lose kinetic energy, and, conversely, it will decrease if the electrons gain kinetic energy.
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