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In contrast, in trans E element has been successfully used to significantly increase the transposition efficiency of the mini-Mu LR) unit.
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Initial stages of formation and expansion of the TIR-220 repeats may have increased the transposition efficiency of Tetris through the advantages explained above.
A comparison of the E-carrier ((E-plus -helper 1) and thE-plus -helperdid not contain thE-plus -helperE-minus)-helper 2) revealed a nearly two-order magnitude of increase in the transposition efficiency of the mini-Mu(LR) units when the (E-plus)-helper was used.
When we increased the amount of transposase by 10-fold, the transposition efficiency of mPB improved 2.4-fold.
With culture treatment, the transposition efficiency was up to 20%.
Yet, the transposition efficiency, specifically in germinal cells, needs to be improved.
Conjugation with pHB14 plasmid and induction of the transposition in S. meliloti produced approx. 3.22 × 104 transconjugant colonies harboring the fluorescent marker with the transposition efficiency of 0.8%.
Several factors have been shown to affect the transposition efficiency of SB transposons in vitro.
The transposition efficiency for pXLBacII-cassette and Tol2ends-cassette is around 1~2%.
Element size, as well as internal structure, can influence the transposition efficiency [ 31, 33, 34].
All four binding sites are required for transposition and, in general, the closer the IR/DRs, the higher the transposition efficiency [243].
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