Sentence examples for increase the transformation efficiency from inspiring English sources

Among the non-viral vectors, nanoparticles showed remarkable properties regarding gene delivery such as the ability to target the specific tissue or cells, protect target gene against nuclease degradation, improve DNA stability, and increase the transformation efficiency or safety.

The surface of leaves was slightly wounded before infiltration to increase the transformation efficiency.

Apart from that, the use of freshly isolated immature embryo or embryogenic calli was also able to increase the transformation efficiency in maize [ 14, 15].

Janna et al. [ 32] also reported that there was no significant difference observed between 1x and 2x bombardments; however, 2x bombardments have been shown to increase the transformation efficiency in banana [ 33], Brazilian maize [ 34], and date palm [ 25].

The presence of sucrose and Mg2+ in HSMG buffer increased the transformation efficiency by 20%, 50 % and 70 over those in HS buffer, glycerol solution, and water, respectively.

For increasing the transformation efficiency, the DNA ligase should be inactivated at 65°C after the ligation reaction was finished, the ligation product need to be desalted by dialysis, and transformed immediately.

We provide a stable S. meliloti hsdR deletion mutant showing enhanced transformation with Escherichia coli-derived plasmid DNA and demonstrate that using an E. coli plasmid donor, expressing S. meliloti methyl transferase genes, is an alternative strategy of increasing the transformation efficiency of S. meliloti.

It can be seen that VirD2 expression during cocultivation slightly increased the transformation efficiency.

If the conspecific DNA uptake sequence (or US, as I refer to them collectively) is present in the donor DNA, it increases the transformation efficiency many fold [ 9].

Deletion of the ChyI restriction enzyme in C. hydrothermalis, a homolog of a HaeIII-like restriction enzyme known to be a barrier to transformation in C. bescii [ 20, 21], increased the transformation efficiency by about an order of magnitude.

Restriction of DNA was not an absolute barrier to transformation, but deletion of the ChyI restriction enzyme in C. hydrothermalis increased the transformation efficiency by an order of magnitude.