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Two affiliative aptamers were used to increase the assay specificity through sandwich recognition.
The problem of using such a kit, however, is that it would increase the assay cost and turn-around time.
We then assessed which combination of these markers could increase the assay sensitivity without substantially lowering its specificity.
An NADPH concentration of greater than 44 μM did not increase the assay performance significantly and so this concentration was adopted.
The use of an auto-puncher and a bar-code system may increase the assay throughput and reliability, although we did not try this, owing to limited resources.
Signal amplification strategies are approaches that amplify the analytical signal generated by each labeled or unlabelled probe so as to increase the assay sensitivity.
Similar(51)
They require many steps or specialized equipment increasing the assay duration and cost.
Haraya et al. [74] confirmed that as the number of lysine residues increasing the assay would be more sensitive.
Further, co-trapped laser-cooled atomic ions sympathetically cool all the ions, increasing the assay phase space density and thus the mass resolution.
Hence, we have designed an alternative protocol to traditional enzymatic digestion of DNA which (i) limits the potential for artifactual oxidation, (ii) speeds up the assay markedly, (iii) increases the assay's sensitivity moderately, and (iv) addresses criticisms that have been raised concerning the efficiency of DNA digestion by nucleases.
This indicates that decreasing the plasma volume in samples increases the assay sensitivity.
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