This is consistent with the mechanisms described in acute hypoxia in the retina [35] and in other tissues, [17], [37] though increased stabilisation of mRNA may be involved in the retinal response to sustained systemic hypoxia [38].
Using computational methods it was determined that the elimination of possible open-chain conformations provides entropic effects enhancing the observed increased stabilisation.
However, the increased stabilisation of mutant protein afforded by the cofactors suggests that their use should be re-examined in some cases.
The rational that promoted this therapeutic approach was that increased stabilisation of Rpn4, a transcription activator of proteasome genes, severely reduced cell viability under stressed conditions.
In order to determine if accumulation of Cx43 at the plasma membrane following incubation with 7-keto was associated with an increased stabilisation of the protein at the membrane, cell-surface proteins were labelled with biotin.
Increased stabilisation of DELLA proteins, repressors of GA responses [ 39], promoted by ethylene signalling via CTR1 may be one of the causes of growth inhibition, which has already been proposed for roots [ 40].
In the tumour microenvironment, hypoxia results in increased transcription of the Glut-1 gene, mediated through HIF-1, and reduction in oxidative phosphorylation, in the absence of hypoxia, leads to increased stabilisation of the Glut-1 mRNA (Behrooz et al, 1997).
In addition to HIF and hypoxia-regulating metabolic processes, metabolic intermediates (including succinate and fumarate) can increase HIF stabilisation via inhibition of prolyl-hydroxylases (Schulze & Harris, 2012).
The substantial Hb increase and stabilisation at 11 12 g/dl in FCM-treated patients suggest a role for i.v. iron alone in anaemia correction in cancer patients.
In the Swiss canton of Vaud (about 730 000 inhabitants), the number of OSTM followed the same trend of increase and stabilisation: since 2000, approximately 1600 patients have benefitted from an OSTM.
