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However, application of anti-repressor elements, which increase promoter activity, did induce the formation of clones that expressed proteins at high levels.
By contrast, CpG islands may be required for transcription factor binding sites, thus the presence of CpGs could increase promoter activity [15].
Whether differences between clades are attributable to SNPs, for example, which increase promoter efficiency or involve unidentified factors, is not known.
It has previously been shown to increase promoter activity of IL4 transcription and was associated with elevated levels of serum IgE in asthmatic families [25].
The CCAAT box has been reported to act cooperatively with HSEs to increase promoter activity [ 25].
Genetic tools are at hand to increase promoter activity and to improve strains easily by targeted knock outs of proteases, random mutagenesis and secretion optimisation.
Similar(41)
Although there were exceptions, at the majority of promoter sites hypoxia did not increase promoter-bound RNApol2 substantially.
We therefore postulated, and have since demonstrated, that specific polyamine analogues can effectively inhibit LSD1 activity, increase promoter-bound levels of H3K4me2 and lead to re-expression of previously silenced genes [ 7, 10].
Clones were ordered from the left to the right according to increasing promoter strength.
By contrast, EpiSCs exhibit increased promoter 5mC coupled with reduced 5hmC, which contributes to their developmental restriction.
Abundance of these sites is indicative of increased promoter strength and correlates with gene transcription levels.
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