Your English writing platform
Discover LudwigSuggestions(1)
Exact(2)
This permitted the generation of combination "helper-independent" piggyBac transposase transposon vectors that exhibited a 2-fold increase of transposition activity in human cells as compared with cells transfected with separate transposon and transposase plasmids.
We cannot discard the existence of a similar mechanism in D. subobscura that was de-repressed as a consequence of the colonization process contributing simultaneously to an increase of transposition of different transposable elements.
Similar(58)
The R-dependent increase of the transposition product from hour 12 to hour 24 (Figure 3B) was likely due to selection against the cells without transposition through restriction enzyme action on them (Figure 1B (i)).
Inclusion of a Gal4 DNA binding domain as an N-terminal fusion to the transposon has been shown to increase the number of transposition events [ 40] which in turn can result in improved protein expression levels.
All strains tested resulted in an increased rate of transpositions (up to five-fold for XXX) compared to the rate in uninfected controls, and all these increases were significant (P < 0.05), except in the case of VXX.
DOI: http://dx.doi.org/10.7554/eLife.00668.012 Increasing the size of the genome in the S-NEC/ASO model increases the rate of transposition in direct proportion.
Addition of mitomycin C, a DNA-damaging agent known to cause chromosomal breakage and induce the SOS response, increased the number of transposition products by four orders of magnitude in the R-negative culture and one order of magnitude in the R-positive culture (Table S4).
This relieves OPI and increases the rate of transposition twofold (not shown).
For example, what is generally observed in plants is a large accumulation of transposon transcripts accompanied by a moderate increase in the rate of transposition and in some cases even no increase (see Ito 2013 for discussion).
They showed that the rate of transposition increases at temperatures below 30°C, which is the optimal growth temperature for Saccharomyces cerevisiae.
A comparison of the E-carrier ((E-plus -helper 1) and thE-plus -helperdid not contain thE-plus -helperE-minus)-helper 2) revealed a nearly two-order magnitude of increase in the transposition efficiency of the mini-Mu(LR) units when the (E-plus)-helper was used.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com