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The oscillation phase of Per1 expression was advanced with the increase of repression intensity; however, it did not occur ahead of Per2 expression (Figure S1 and Text S1; see discussion).
A possible increase of repression by PER/CRY complexes due to constitutively high levels of PER1 (Fig. 5H,I) would be expected to lengthen the circadian cycle, consistent with the longer free-running period of Usp2 KO mice observed in our running-wheel experiments.
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For this circuit the mechanism is well understood: an excessive increase of the repression strength (while potentially improving the noise reduction of the circuit) reduces the copy number of mRNAs and proteins with a consequent rise in intrinsic fluctuations (which can overcome attenuation).
China's north-western area of Xinjiang will double its anti-terrorism budget in the coming year, state media have reported, raising concerns of an increased wave of repression in the ethnically divided region.
Already, there have been increased reports of repression against dissenters.
Since Enrique Peña Nieto became President and Miguel Angel Mancera took office as Mexico City´s Head of Government in December 2012, there have been increased incidents of repression of protesters and silencing of journalists — who are attempting to document these aggressions and the violence that is afflicting the nation.
"The one left is a constant increase in levels of repression and violence, and I think that will fail, too.
The binding of multiple miRNAs to a single target mRNA can increase the effectiveness of repression [40].
Follow up studies on CRISPRi showed that the fusion of repressor effector proteins such as the KRAB (Kruppel-associated box) domain of Kox1 to dCas9 could increase the effectiveness of repression of reporter gene activity [ 59].
We further showed that sumoylation increased the extent of repression (Fig. 5D).
Co-expression of two different miRNAs did not increase the repression of the reporter gene, indicating no additive or synergistic effects between the tested miRNAs.
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