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If we consider the gene expression profile at 6 hours after inoculation (Additional File 2), we first notice the rapid increase of motif candidates that passed the expression significance filter.
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Since the increase of network motif size boosts the computational time and the number of motifs exponentially, we set the size of subgraphs as four to five for practical experiments.
Nevertheless, the GPY/F module relates the Retroviridae class I with Ty3/Gypsy LTR retroelements of plants and fungi through the common preservation of the canonical motif, while classes II and III can be related with bilateria Ty3/Gypsy LTR retroelements by an increase of the motif variability.
Therefore, pattern-driven algorithms are feasible for small motif length l (l ≤ 20), but they will take long running time or have high space requirement with the increase of the motif length.
Interestingly, the progressively increasing trend of motif frequency toward gene ends does not keep in the subregions that are immediately adjacent to gene ends (e.g., the interval 90 99 in Figure 5A and the interval 0 9 in Figure 5B).
The newborn motifs would subsequently increase the incidence of mispairing, which in turn would increase the frequency of motif indels.
Motif-X also computes a fold increase of the kinase motif in the sample by determining the total number of motifs found in the entire rat database.
Analysis of the secondary-structure context in which these motifs are embedded might provide additional biological information that could increase the specificity/sensitivity of motif detection.
The medium of decoration and an increased range of motifs felt appropriate for tomb decoration led to the introduction of small, often entertaining details into standard scenes.
The combinatorial nature of the deterministic mining process may deliver an exponentially increasing number of motifs.
Consistent with the EMSA results, neither WT nor mutant KLF4 constructs showed any significant increase of transcription when motif M412 was methylated in the construct.
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