Exact(5)
These mutants show a strong increase of Km and some decrease in turnover rate against MpNPT (Fig. 4a).
The increase of KM by over 10 times was determined for polypyrrole-coated GOX, if compared with native GOX.
On the contrary, change at position 274 of Phe instead of Ala (mutant A274F) caused a significant increase of Km values for NAD(P) and for NADPH and even a more marked decrease in catalytic activity.
Both residues at the active site of mAOX1 seem to be important for the binding of substrates, since the M884R variant either completely lost the activity with phtalazine or acetaldehyde as substrates, or the activity with retinaldehyde and benzaldehyde was drastically reduced, with a concomitant increase of Km for benzaldehyde.
The catalytic efficiency (kcat/ Km) while using the non activated substrate cellobiose was from 44 to 1026-fold lower than that found for the best pNP substrate, mainly due to a significant increase of Km values (from 7 to 260-fold, depending on the enzyme) for the disaccharide.
Similar(54)
Denmark's purchase-tax system led to an average fuel efficiency increase of 4.1 l/100 km for diesel light vehicles and 0.6 l/100 km for petrol [74].
Using the SAS scores as predictor suggest an average fuel consumption reduction of 1.21 · (−0.90) L/100 km = −1.09 L/100 km or −2.67 % for those subjects answering with "yes" on the judgment of learning and an increase of 0.4 L/100 km (0.97 %) for the subjects answering in the negative.
While diabetes significantly decreased the brain specific activity (Vmax) of AChE soluble form by 42%, it caused a fivefold increase of the Km of the membrane-bound form.
Whilst the magnitudes of the effects of temperature and wind speed in model 1 appear small, it should be noted that these conditions have a considerable range – for example an increase of one km per hour in wind speed overall decreases robbery by 1.1percentnt.
An increase of 1 km in the home range radius increases the risk of having rinderpest by 1.6%.
As expected, this polymer concentration produced an inhibition close to the competitive type, that is, an increase of apparent Km and no change of Vmax.
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