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The intakes and total apparent digestibilities of DM, OM, CP and NFC and TDN intakes increased linearly (P < 0.01) with the increase of concentrate in the diet.
On the other hand, there was an increase in population of R. amylophilus (0.012 vs 0.021%, P = 0.07) in response to the increase of concentrate in the diet.
Furthermore, the increase of concentrate in the diet, from 60 to 80%, resulted in a significant reduction in the population of S. bovis (0.022 vs 0.004%, P < 0.01).
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Generally, average daily gain increased with increasing levels of concentrate supplementation.
Other studies found similar results, where the population of S. bovis was not increased with the increment of concentrate in the diet of Nellore steers, using corn silage as the roughage source (Granja-Salcedo et al. 2016) or freshly-cut sugarcane (Ribeiro Junior et al. 2016).
Purine derivative excretion in the urine increased with an increasing proportion of concentrate in the diet, and there was a strong linear relationship between total PD excretion (allantoin and uric acid) and microbial N flow to the duodenum: microbial N (g/d) = 19.9 + 0.689 × total PD (mmol/d); R = 0.887.
Boron contamination of permeate in seawater RO desalination is commonly prevented by increase of the concentrate pH level in a rather elaborate multi-pass processing system.
The results showed that with the increase of treatment concentrate of SO2 derivates (a mixture of sodium sulfite and sodium bisulfite, 3 1, mmol L−1/mmol L−1.0 5.05.0 mmol L−1), the physiological activity of the guard cells declined and cell death occurred.
In addition, the increase of dietary concentrate increased the population of R. amylophilus and reduced F. succinogenes, R. albus and S. bovis, highlighting the hypothesis that S. bovis has a higher affinity for sugar (Additional file 1) than the for starch itself.
Consequently, concentrate CP content decreased with the increasing level of concentrate.
The effect of D-value on SDMI diminished with increasing level of concentrate supplementation (interaction P<0.001).
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