All the tested pyrazole derivatives induced a concentration-dependent increase of cells in G2/M phases.
Within 24 h after RAD001 administration, a significant increase of cells in G1 phase has been described [35, 36].
Incubation of a rat VSMC (A10 cells) with OA (50 μM) resulted in an increase of cells entering the S phase of the cell cycle.
Contrary to breast cancer cells, there was a small increase of cells at S phase, which may indicate a promotion of cell proliferation in view of the higher number of cells synthesizing DNA.
With increase of cells implanted, the dark signal became more and more intense, and cells treated with SHU555A could only be detected at cell number of 1 × 106 (Figure 8C).
As expected, growth medium led to a significant reduction of cells in G0/G1 (65.1% compared to 91.1%) and an increase of cells in S phase.
In this population, we also observed a strong increase of cells with less than 4 centrioles (43.8±15.6%) compared to controls (13.8±4.9%).
The increase of cells at G1 phase and decreased fraction of replicating cells could explain the low toxicity of radiomimetic treatment with etoposide and camptothecin.
Data from the flow cytometry analysis of surface bound protein further show an increase of cells positive for amphiregulin (Figure 3C).
Interestingly, the increase of cells in S-phase and M-phase was maintained along the entire antero-posterior axis of the VM.
In addition, B cells also demonstrated a significant increase of cells expressing the activation markers CD5, CD40, CD95 between early and late (data not shown).
