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However, the increase of AGEs level in serum was obviously depressed by the administration of ES and AG.
In addition, accelerated increase of AGEs accumulation may be determined in an inflammatory milieu.
With the increase of AGEs concentrations, the presence of autophagic vacuoles became progressively prevalent.
With the increase of AGEs concentration, the cell viability decreased progressively and reached a lowest level at AGEs 100 mg/L.
Clinical study has also observed significant increase of AGEs accumulation in diabetic vascular tissues [ 25], which may induce macrophage migration across an endothelial cell monolayer [ 26].
To elucidate whether RAGE responded to the increase of AGEs from ribosylation, western blotting was used to detect cellular levels of RAGE under the experimental conditions.
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