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Increase in buds and bud scars implies that compound affect normal division process of yeasts resulting in a single cell with multiple attempt to divide but not resulting in increase in viable number of cells (Watanabe et al. 2006).
Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO2 laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000 cells/ml when compared to the as-received sample.
When stimulated through CD137 ligand murine monocytes responded just as human monocytes with an increased adherence, morphological changes, proliferation and an increase in viable cell numbers.
For example, at 48 h the fold increase in viable cells was 29-fold (±2.5) compared to 86.5-fold (±2.8) at 96 h.
We observed the presence of phages in the biofilm at all time points, indicating that spontaneous phage induction is occurring continuously and paralleling the increase in viable cells.
Adaptive regrowth could explain the relative increase in viable cells detected at later time points in populations of cells ectopically expressing CLN3, despite an increased frequency of apoptosis in these cell populations (Fig. S4).
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MRI results after 6 months showed reductions in scar mass, increases in viable heart mass and contractility in the treated group [69].
Altered levels of sphingolipid species have been found between tumour and normal tissues and the lipid species (m/z 725) shown here has been known to be increased in viable tumour tissue [22].
At 48 h, total viable cell number and fold-increase in viable cells was similar to that observed in batch culture.
However, at 96 h there was a significant increase in both total viable cell number and fold-increase in viable cells, suggesting the cells undergo an adjustment phase in the initial 48 h of culture.
Thus, the initial anti-adhesive effects of NeoA were not likely caused by cytotoxicity, which was expected given that our initial anti-invasion screen was designed to detect increases in viable, non-invasive cells after three hours of compound treatment (10, 15).
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