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In addition, 28 subjects with pre-existing rHuPH20-reactive antibodies prior to exposure experienced treatment boosting, as defined by an increase in titer of two titering steps or more following treatment, and one of these experienced a maximum titer of 81,920, the highest titer observed following rHuPH20 exposure in this data set.
To discern the regions necessary to achieve this 10,000-fold increase in titer, additional constructs were designed and tested.
A significant increase in titer of circulating antibodies to the bovine cells was observed after 4 days in all animals implanted with xenogeneic cells.
No significant increase in titer of antibodies to the porcine cells was observed during the time course of the experiment in animals implanted with porcine endothelial cells.
Specifically, we utilize this approach in an iterative fashion for the glycerol biosynthesis pathway and ultimately achieve a 5.7-fold increase in titer.
A combination of the pathway engineering and optimal culture parameters led to a 37-fold increase in titer to 2.2 g/L and a 50-fold increase in yield to 0.13 (g/g glucose).
An increase in titer was noted 24 hours after inoculation, and normal virus maturation was documented by ultrastructural study of the infected cells.
A fourfold increase in titer is more difficult to achieve if the baseline titer is already high.
"Seroconversion" was defined as a four-fold or greater increase in titer between pre-vaccination and post-vaccination sera.
This increase in titer is not understood, but could either be an effect of the H5N1 HPAI virus challenge or related to the timing of the original immune response (31 LPAIV-dpe).
Almost all participants had some detectable increase in titers, with 96% (43/45) having a two-fold or greater increase in titer by HI compared to 98% (43/44) by VM.
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