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Interestingly, though GRP receptor expression has not been reported in the DMH, WT DMH cultures showed an increase in rhythm amplitude in response to GRP treatment (p<0.05; Table 2, Fig. 4C) while Vipr2−/− DMH cultures failed to do so.
In comparison with PC recorded in BK−/− mice, PC recorded in WT following paxilline injection presented a similar increase in rhythm index (Fig. 7E), but an increase rather than an increase in simple spike frequency, and no difference in complex spike firing rate and duration (Fig. 7G,H).
44, 45 The power spectrum revealed that there was a broad increase in rhythm power at the lower frequencies, between 2 and 18 Hz post-Met treatment (Fig. 5a), which encompasses the theta and alpha rhythms.
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More robustly rhythmic tissues (WT SCN, WT and Vipr2−/− pituitary gland) did not show significant increases in rhythm amplitude following forskolin treatment (Table 2), presumably as these tissues had not yet significantly damped at the time of treatment and/or did not suffer any inherent rhythm abnormalities associated with a lack of VPAC2 receptors.
Forskolin treatment induced robust increases in rhythm amplitude in tissues that previously showed either a circadian deficit due to altered VIP-VPAC2 signaling (Vipr2−/− SCN; p<0.005; Table 2), or demonstrated rapid damping of oscillations (WT and Vipr2−/− Arc/ME/PT, p<0.005 and p<0.0005, respectively; WT and Vipr2−/− DMH, p<0.01 and p<0.005, respectively; Table 2).
These start with an advance in the timing of core body temperature rhythms that is present by 4 months of age, and progress to include increases in rhythm amplitude and overall mean body temperature by 6 months of age.
Many are linked to an increase in heart rhythm, known as torsade de pointes, that can lead to death.
In this regard, it should be borne in mind that the accumulated burden of migraine seems to cause slight alterations in the physiology of the visual cortex and an increase in alpha rhythm variability up to 72 h before the next migraine attack [58].
We found that methyl supplementation with Met resulted in a marked increase in theta rhythm power.
We, therefore, hypothesize that another benefit of methyl supplementation is a reduction in interictal spike activity and an increase in theta rhythm power that may help promote proper memory consolidation in epileptic animals.
Recently, there has been an exponential increase in developmental mu rhythm research, partially due to the mu rhythm's potential role in our understanding of others' actions as well as a variety of other social and cognitive processes (e.g., imitation, theory of mind, language).
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