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We observed a 10-fold increase in neutralization efficiency of rVSV-EBOV GPΔβ17 β18 compared to GPWT (Fig. 3c).
These results suggest that changes outside the V3 loop might also have contributed to the increase in neutralization sensitivity to anti-V3 mAbs.
It is important to mention here that this increase in neutralization capacity was not due to antibody isotype switching (Figure 3E), which was consist with previous studies [24].
Paired t-test was performed to calculate their increase in neutralization after booster vaccination and linear regression was used to calculate influence of circulating antibodies on booster vaccination.
Of the four patients (ITM60, NYU104, 3506 and ITM27) whose sequential viruses exhibited increased sensitivity to neutralization by anti-V3 mAbs, those from patient ITM27 exhibited the weakest increase in neutralization sensitivity (Figure 2h).
Previous studies with other viruses have shown that combination of two or more than two mAbs directed against different epitopes could lead to a two to ten fold increase in neutralization titers [28], [29] and provided greater protection against many other diseases [30], [31], [32].
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The low IgM titres in this gnotobiotic pig might correspond to the slight increase in PCV2-neutralization (up to 16% whereas in other pigs they reached 97%) that was observed in this pig.
The mean increase in percentage of neutralization by serum (dilution 1∶16) before and 2 weeks after vaccination in the i.d.
In contrast, the antibody response showed significant increase in the virus neutralization capacity by day 75 post-immunisation (Figure 3C) which was co-incident with the antibody avidity maturation (Figure 3D).
Interestingly, despite the presence of significant cross-reactive neutralization activities, 1 dose of 2009 H1N1 vaccine resulted in a significant increase of neutralization activities in 21 out of 23 volunteers.
Neutralization titers, measured at week 5 after immunization, of ≥640 were observed in AdHA.cod and in some AdHA.wt immunized animals, again with a significant increase in geometric means of neutralization titers observed after AdHA.cod immunization.
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