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Because the functional relevance of induced gene expression needs to be confirmed by a subsequent increase in expressed protein levels, we analyzed six of the investigated marker genes (Hmox1, Ptgs2, Cyp1a1, Gsta1, Hsp1a1, and Nqo1) on the protein level (HO1, COX2, CYP1A1, GSTYa1, HSP70, and NQO1) by immunoblotting of cytoplasmic extracts from lungs 24 hr after particle instillation.
The feed strategy with these 4 supplements led to a significant increase in expressed r-SAK-2 (Table 1).
However, the substantial increase in expressed in vivo genes at 168 hrs was attributed to triglyceride accumulation and immune cell infiltration, which was not observed in Hepa1c1c7 cells.
In a pre-post comparison (Hypotheses 4) the children of the two ethnic groups showed a significant increase in expressed emotions in the doll play narratives.
In all three tissues characterized in this study, HMGB1 showed a 2-fold or greater increase in expressed transcripts when acclimated to elevated temperatures.
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Two studies, one in Australia and the other in Singapore provide the only data actually documenting an increase in expressing over time [ 2, 6].
These two independent analytical techniques consistently found an increase of ∼80% in expressed hydroxyapatite when compared to a biomedical titanium alloy.
The fold increase in genes expressed in response to type I IFN was generally twofold higher than the fold increase for genes expressed in response to SAV-3 infection.
Arithmetic is cruel to currencies in decline: this corresponds to a 30 per cent increase in prices expressed in sterling – assuming zero inflation in the destination country.
Over time, the background fluorescence decreased as a result of rapid clearance of the probes, which led to an increase in contrast expressed as T/B ratios.
The increase in differentially expressed genes indicated increased variability when reducing sample size.
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