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CSPG-treated cells generated fewer colonies between 0.5 and 0.8 mm in diameter, with a significant increase in colonies between 0.8 and 1.2 mm as compared to the control (Fig. S3A).
The significant increase in colonies seen with the Spy1-TST mutant over Spy1-WT supporthathathehe G2/M degradation mechanism previously described [ 11] may provide a protective barrier against this potentially oncogenic pathway.
(D ) The fold-change in colony counts of transformed wild-type (WT) (n = 22 replicates) and Craf −/− (n = 14 replicates) MEFs demonstrate a dose-dependent increase in colonies, particularly for WT MEFs.
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Oct4-overexpressing ATSCs induced a 1.5-fold increase in colony formation with increased synthetic DNA and telomerase activity along with slightly extended telomere lengths (Fig. 1D).
The growth kinetics at 25°C (i.e. increase in colony diameter per measurement) were also similar for these strains.
Transfection with total cellular RNA isolated from HCV1a/PAC replicon-containing cells led to a significant increase in colony-forming ability.
In this assay, mean revertants and fold increase in colony numbers were observed in a dose dependent manner with increasing concentrations of tobacco smoke and particulate deposition.
Colony size increased significantly with time, although there was no significant increase in colony size between days 19 and 22, days 12 and 15, and days 8 and 12.
With the UAMH strains, the controls (6.6 ± 2.6 mm) did not differ from tree of heaven, spalted aspen, or aspen; only sugar maple (8.9 ± 4.6 mm) caused a significant increase in colony diameter over the control.
The increase in colony size diversity indicates the evolution of phenotypically measurable genetic divergence within microcosms.
At 96 hours, we observed a dramatic increase in colony diameter.
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