However, deregulation beginning in the 1960s led to a substantial increase in branch banking and bank account holders, and legislation in 1984 further reduced controls over banks' activities, which thereby enabled them to offer a wider range of services and led to greater competition.
This increase in branch surface area occurs with age and increase in size and numbers of branches at the same time that the amount of potential storage volume to support water loss from this expanded surface area is also increasing.
Consistent with previous experiments, statistical analysis of the result showed that microglia induced a 5-10 fold increase in branch number (p<0.0001) (Fig 4D).
To ascertain whether the increase in branch number and reduction in AZs resulted from a failure to coordinate growth of the presynaptic motor neuron and postsynaptic muscle, we examined wnt2 mutant NMJs during both 1st and 2nd instar larval stages (24 and 48 h AEL, respectively).
P. porites was sectioned down the growth axis (i.e. from base to tip of the branch), and we measured linear extension of the skeleton (i.e. increase in branch length) as the length of coral skeleton distal to the alizarin red band.
Quantification revealed an almost twofold increase in branch numbers at E14.5 and a 30% increase at E15.5 (Fig 7Ci).
