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These results were confirmed by the study of the amount of Fas and Fas L bound to the cell surface by flow cytometry (PE fluorescence), showing an increase in bound protein in both cases.
This increase in bound LC3 on Atg3 diminishes the likelihood of both enzymes in a complex having catalytic cysteines free to form a disulfide.
Although hypoxia is associated with a decrease in free ribonucleic acid polymerase II activity, we observed a marked increase in bound ribonucleic acid polymerase II activity, which may represent a hypoxia-induced alteration of gene expression.
However, for heating times of 20 min, a significant increase in bound protein mass occurs.
As shown in Figure 4D, a semi-quantitative analysis based on three experiments indicates that there is, on average, a four-fold increase in bound PARP-1 and a six-fold increase in bound Ku80 at the HEK293 (CA)12 site relative to the HeLa (CA 11 site.
Given the low cytosolic to nuclear zinc ratio and unchanging cytosolic FluoZin-3 staining, we postulate that the increase in bound nuclear zinc results from import of extracellular zinc as opposed to mobilization of cytosolic sources.
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Corporate America has $3 trillion ready to invest, waiting for the President and Congress to determine the increase in revenues bound to occur.
Reaction with Ferene-S, a dye that forms a blue reaction product with iron [25], shows a marked increase in protein bound iron in all cell lines compared to unexposed controls (Fig. 5B).
Incubation with the cognate ligands lead to a significant increase in membrane bound radioactivity in comparison to control membranes, indicating that ligand binding to OR22a is indeed activating the G-protein (Figure 4E).
On the contrary, a decrease in T2rt reflects an increase in protons bound to macromolecules.
An increase in nonmembrane bound organelles and factors related to biogenesis and membranes were also reported.
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