Using a SSTR2-based reporter system, we demonstrate that angiotensin II can be used to increase gene expression upon intra-arterial adenovirus delivery.
In the present study, we examined whether introduction of CS into PPC containing five cationic amino acids can increase gene expression in tumor cells.
In the present study, we examined whether introduction of CS into pDNA/PPC complex can increase gene expression in tumor cells.
Smart multifunctional nano-carriers can be engineered with tunable physicochemical properties to increase gene expression and enhance cellular uptake, including organelle-specific escape and nuclear membrane targeting.
Although ultrasound has been shown to increase gene expression 10 15 fold in vivo compared to "naked" DNA [39], levels of gene expression are still considerably lower than that which can be achieved using either EP or gene gun approaches [39].
To examine whether introduction of CS into pDNA/PPC complex can increase gene expression in tumor cells, pDNA/PPC (charge ratio = 1 2) and pDNA/PPC/CS (charge ratio = 1 2 16) complexes were transfected into B16 melanoma and HepG2 cells.
Re-stimulation with T. borreli did not increase gene expression of p35 nor p40a.
Moreover, resveratrol was independently able to increase gene expression including glutathione peroxidase, keratin 1 and oxidation resistance 1 and decrease the expression of ribosomal protein L13a (Table S1).
By combining Cre-lox technology with the use of two well-characterized 3'UTR sequences, c-Fos-3'UTR and bGH-3'UTR, a single allele that can decrease or increase gene expression has been assembled.
To uncover the potential "hidden' attractors that may resemble that of differentiated cells, we hold Pdx1 gene expression to be zero on average but increase gene expression amplitude for the stochastic fluctuations.
Nevertheless, it is likely that increasing the level genome-wide of histone acetylation through inhibition of histone deacetylases inducing non-specific chromatin opening would increase gene expression noise due to the random activation of previously repressed genes that would overweight context-dependent noise.
