In this study we also identified 80 transcripts as potentially antisense-specific, although further studies would be needed to confirm this because of the possibility for incorrectly oriented probes or strand bias during hybridization.
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Some of incorrectly oriented sequences can be detected by looking for alignments in the original direction having multiple non-consensus splices, that become all-consensus, high-identity alignments when reversed and complemented.
Asymmetric GSC division is further ensured by the centrosome orientation checkpoint (COC) that prevents mitotic entry in the presence of incorrectly oriented centrosomes (Cheng et al., 2008; Inaba et al., 2010; Yuan et al., 2012).
He never said it was a Diebenkorn, but along with a picture of the entire painting -- incorrectly oriented vertically, which he noted in his posting -- he placed a closeup of the hole.
In each case, the failure to connect is often accompanied by excess incorrectly oriented vein growth.
The eyes, though shifted medially and incorrectly oriented, were present and their gross morphology conserved in ablated mutants (Fig. 1I, J).
It is possible the BAC assembly incorrectly oriented the sequences corresponding to Fig 1a Hit iv because the Genscan analysis discovered this region coded for the C-terminal region of Cyp41, while the Figure 1a Hit v sequence coded for the N-terminal and middle areas of Cyp41 (Additional file 1).
We demonstrate BAIT can refine completed reference assemblies, identifying approximately 21 Mb of incorrectly oriented fragments and placing over half (2.6 Mb) of the orphan fragments in mm10/GRCm38.
We showed previously that Strand-seq also has an application in correcting incorrectly oriented portions of the mouse reference assemblies [ 1].
