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A "dirty" WB or incorrect localization in IF or immunohistochemistry (IHC) warns of potential non-specific binding in ChIP assays, he says.
If an antibody shows incorrect localization in IF or IHC or both, then cross-reactivity in a ChIP assay becomes more likely, he says.
Highly similar flux solutions were computed using either a model with undefined pathway localization or a model with constraints based on curated (S. cerevisiae) or computationally predicted (H. polymorpha) localization information, while false solutions were found with incorrect localization constraints.
However, this makes it difficult to evaluate if incorrect SISCOM localization was truly incorrect or if it was due to incorrect localization of the seizure focus by other modalities.
This connection might also mean that if the observed increased expression of LDLR in apparent compensation for incorrect localization induced by APP overshoots, then more apoE-cholesterol could be imported, leading to more APP processing to Aβ in an accelerating pathogenic cycle.
Though FLNa is crucial in the initial step of CCL2-induced vesicle formation, we have also observed the presence of activated receptor and FLNa in trafficking endosomes, and the incorrect localization of late endosomes in cells lacking FLNa which suggests that it may also be involved in other steps of CCR2B-vesicle trafficking besides the initial budding step of endosomes.
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Table 1 shows the average accuracy results of each landmark point as well as the average distance from the ground truth position for both correct and incorrect localizations.
It is difficult to determine the exact number of incorrect localizations to pseudogenes as relatively few mouse pseudogenes have been annotated.
In contrast, use of an unmasked version of the genome results in 100% recall for the test set of sequence tags, but increases the number of incorrect localizations by as much as ten-fold.
It is also possible that incorrect auxin localization, due to abnormal PIN protein function, in turn causes defective vascular patterning.
A SUMO-deficient DRIL1 mutant is not subject to incorrect subcellular localization and retains DNA-binding capability comparable to wild-type DRIL1.
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