Exact(1)
The incorporation values of FA-PLP-131I at 6 h were 3.12- and 23.4-fold higher than those of 131I and PLP-131I, in agreement with the results of confocal laser scanning microscopy images of B16F10 cells incubated with fluorescein isothiocyanate-labeled FA-PLP-131I and PLP-131I nanoparticles (Additional file 1: Figure S1).
Similar(59)
We assume a double exponential distribution around the true number of incorporated nucleotides, i.e. the probability density of observing the incorporation value o i when the true number of incorporations is f i and the flow cycle is i is given by: Note the non-standard parameterization using the standard deviation instead of the usual rate parameter.
We corrected incorporation values to the amount of DNA present in each culture to provide an index of DNA synthesis per cell (Winick and Noble 1965), and we recorded the total DNA content.
FA-PLP-131I, as well as FA-PL-131I, showed the higher incorporation values than that of all tested time points, increasing with time.
The resulting read is then specified in terms of a flowgram a sequence of incorporation values, one for each flow.
However, by observing the whole sequence of incorporation values together, we would have been able to deduce that the fourth incorporation of 0.4 should be rounded up, not down.
Having fully defined the likelihood function (given a set of noise model parameters θ), we now seek to find the series of true incorporation values (F) that would maximize the likelihood of the observed incorporation values (O).
A major player is Ion Torrent, a pyrosequencing-like technology which produces flowgrams – sequences of incorporation values – which are converted into nucleotide sequences by a base-calling algorithm.
Incorporation values were corrected to the amount of DNA present in each culture to provide an index of DNA synthesis per cell (Winick and Noble 1965).
Incorporation values were corrected to the amount of DNA present in each culture to provide an index of macromolecule synthesis per cell (Winick and Noble 1965).
Incorporation values were corrected to the amount of DNA present in each culture to provide an index of macromolecule synthesis per cell.
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