Exact(3)
We assume a double exponential distribution around the true number of incorporated nucleotides, i.e. the probability density of observing the incorporation value o i when the true number of incorporations is f i and the flow cycle is i is given by: Note the non-standard parameterization using the standard deviation instead of the usual rate parameter.
The PAPST1+2-KD cells showed the lowest incorporation value, indicating additive effect from reduction of both PAPST1 and PAPST2.
The indicator functions, in the second line of the equation, are used to determine whether the state transition dictates an incorporation event, and whether the incorporation value f i matches the incorporation value dictated by the state transition.
Similar(57)
FA-PLP-131I, as well as FA-PL-131I, showed the higher incorporation values than that of all tested time points, increasing with time.
After 0.5, 1, 2, 4, and 6 h incubation, the cells were washed with PBS three times and their radioactivity measured with a gamma counter (Science and Technology Institute of China, Jia Branch Innovation Co., Ltd .. Incorporation values were calculated as described in the previous literature [25].
The incorporation values of FA-PLP-131I at 6 h were 3.12- and 23.4-fold higher than those of 131I and PLP-131I, in agreement with the results of confocal laser scanning microscopy images of B16F10 cells incubated with fluorescein isothiocyanate-labeled FA-PLP-131I and PLP-131I nanoparticles (Additional file 1: Figure S1).
Let denote the observed (normalized and phase-corrected) incorporation values, with being the true (noiseless) values in flow-space.
Having fully defined the likelihood function (given a set of noise model parameters θ), we now seek to find the series of true incorporation values (F) that would maximize the likelihood of the observed incorporation values (O).
The resulting read is then specified in terms of a flowgram a sequence of incorporation values, one for each flow.
Incorporation values were corrected to the amount of DNA present in each culture to provide an index of DNA synthesis per cell (Winick and Noble 1965).
Incorporation values were corrected to the amount of DNA present in each culture to provide an index of macromolecule synthesis per cell (Winick and Noble 1965).
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