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A model drug incorporation using lidocaine is successful when amphiphilic chitosan nanospheres were dissolved in good solvent followed by allowing mixing with drug solution before dialysis.
The scaffolds were then implanted into dorsal skinfold chambers of C57BL/6 wild-type mice to analyze their vascularization and incorporation using intravital fluorescence microscopy, histology and immunohistochemistry. Scaffolds seeded with differentiated spheroids exhibited a markedly impaired vascularization.
In this research, an asphalt mixture was functionalized with nano-TiO2 by bulk incorporation, using the contents 3 and 6%, and by spraying coating using an aqueous solution over its surface at different temperatures and rates.
Commercial nanocomposite food packaging in the form of nano-silver containers and coated films were tested in real-food matrices to determine the degree of engineered nanoparticles (ENPs) incorporation using inductively coupled plasma-mass spectrometry (ICP-MS) and atomic absorption spectrometry (AAS).
Amplification was tracked via SYBR-Green (PE Biosystems) incorporation using an ABI-PRISM 7700 Sequence Detection System (Applied Biosystems).
Cell proliferation was tested by staining for BrdU incorporation using specific anti-BrdU antibody and FACS analysis.
Cell proliferation analysis was done by immunostaining for BrdU incorporation using a Cell Proliferation kit (Amersham) following the manufacturer's protocol.
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Therefore, throughout this article, the terms "OH-incorporation", "hydrogen incorporation", "proton incorporation" and "water incorporation" are used synonymously.
Therefore, enhancing the Mg incorporation by using the surface effect should be practically feasible.
This value was converted to pmol thymidine incorporation cell−1 hr−1, using the mean direct count data (Table S1).
Radiolabelled DNA was harvested and [3H]thymidine incorporation measured using a β-scintillation counter.
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