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The results of the p-NBA S-HYNIC incorporation test showed a respective ratio of S-HYNIC/CZP of 1.2, 1.5 and 2.0.
In addition to the experiments above, we confirmed the effect of AA on cell division using a BrdU incorporation test (data not shown).
For BrdU incorporation test, sections were pretreated for 20 min with 0.1% trypsin in PBS at 37°C following the 30 min incubation in 2 N HCl at 37°C for DNA denaturation.
Cytostatic effects were evaluated by the tritiated thymidine incorporation test.
The bromodeoxyuridine/propidium iodide incorporation test showed similar results (data not shown).
The Salmonella mutagenicity plate incorporation test was performed according to the method described by Maron and Ames [ 30].
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Similarly, the Salmonella mutagenicity assay, which was developed initially as a spot test (Ames 1971), then as a plate-incorporation test (Ames et al. 1972) using strains of Salmonella bacteria derived from studies by B.N. Ames and P.E.
From the results of incorporation efficiency test, it was found that RPEn cells have a significantly higher attachment affinity to hepatocytes than human dermal fibroblast and rat liver epithelial cells.
Comparison of the T7 RNA polymerase transcription activities for [Pt(dien)(N7-5′-GTP)] compound incorporation reaction test, with respect to the effect of a decreasing concentration of natural GTP, showed no major differences.
The first one uses the standard plate incorporation Ames test, but with a new negative control system.
Items included covered patient spectrum, reference standard, disease progression bias, verification bias, review bias, clinical review bias, incorporation bias, test execution, study withdrawals, and indeterminate results.
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