Exact(5)
Although d-alanine incorporation systems for LTA are generally very specific, we nonetheless addressed the second theoretical possibility that d-alanine analogues might label this biopolymer.
pAzF has been successfully incorporated into other proteins using this optimized pEVOL system and other unnatural amino acid incorporation systems similar to pEVOL.
There are different haem incorporation systems in different organisms [ 37], however, and the non-covalent incorporation observed might be due to the inability of E. coli to recognize the haem attachment site in the mammalian sAC-HD protein.
Furthermore, by understanding the underlying principles for phosphoserine levels in the cell, we may gain important insight into controlling other phosphorylated amino acids in E. coli for future development of orthogonal pThr and pTyr incorporation systems.
We thus assume that one haem cofactor binds per sAC-HD monomer, and that the cofactor is a non-covalently bound b-type haem, although the different haem incorporation systems of E. coli and mammals render the latter conclusion uncertain (see the Discussion).
Similar(55)
We thus sought to obtain a more efficient nitroTyr incorporation system.
The distribution of the selenocysteine incorporation system in different bacteria has been analyzed previously [ 89].
Although the present unnatural amino acid incorporation system is designed for bacteria, new systems have been developed for other organisms.
This substitution improves enzyme characteristics [ 1], such that one in four prokaryotic genomes carries a selenocysteine (SeCys) incorporation system [ 2, 3].
Quality improvement activities and the incorporation of systems (e.g., information systems, care management, work flow redesign) in large group practices (>20 physicians) are also well studied, [ 20- 22] and highlight the resources and approaches needed to overcome barriers to quality improvement, including a lack of staff, resources, and time [ 20- 22].
Additional benefits include better incorporation of systems theory thinking, ease with which team multidisciplinarity and diversity can be identified, and seamlessly integrating design and research.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com