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We thus sought to obtain a more efficient nitroTyr incorporation system.
The distribution of the selenocysteine incorporation system in different bacteria has been analyzed previously [ 89].
Although the present unnatural amino acid incorporation system is designed for bacteria, new systems have been developed for other organisms.
This substitution improves enzyme characteristics [ 1], such that one in four prokaryotic genomes carries a selenocysteine (SeCys) incorporation system [ 2, 3].
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pAzF has been successfully incorporated into other proteins using this optimized pEVOL system and other unnatural amino acid incorporation systems similar to pEVOL.
Although d-alanine incorporation systems for LTA are generally very specific, we nonetheless addressed the second theoretical possibility that d-alanine analogues might label this biopolymer.
There are different haem incorporation systems in different organisms [ 37], however, and the non-covalent incorporation observed might be due to the inability of E. coli to recognize the haem attachment site in the mammalian sAC-HD protein.
Furthermore, by understanding the underlying principles for phosphoserine levels in the cell, we may gain important insight into controlling other phosphorylated amino acids in E. coli for future development of orthogonal pThr and pTyr incorporation systems.
We thus assume that one haem cofactor binds per sAC-HD monomer, and that the cofactor is a non-covalently bound b-type haem, although the different haem incorporation systems of E. coli and mammals render the latter conclusion uncertain (see the Discussion).
As a result, it is undoubtedly important to guarantee accurate prediction of the behaviour of the PDN during transient conditions and its incorporation in system analyses for electric power transmission.
This method is suggested as an alternative to parameterization of traditional models (e.g., Nikolsky Eisenman), for which parameters are strongly dependent on both analyte concentration and temperature, and to standard ANN models which have no mechanism for incorporation of system knowledge.
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incorporation model
transposition system
incorporation governance
participation system
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addition system
involvement system
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constitution system
enactment system
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opportunities system
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shuffling system
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