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O incorporation studies demonstrated that one atom of molecular oxygen was incorporated into each carboxylate product, in accordance with the enzymatic mechanism of Ni II -ARD [142].
The retrobiosynthetic concept was designed to improve the stringency of isotope incorporation studies.
Precursor incorporation studies indicated that it is derived from acetate, l-phenylalanine, l-leucine and l-cysteine.
In the course of oxygen incorporation studies, benzene oxide experiments as well as kinetic studies, H2O2 was quickly added with a pipette (one-time addition of 1 mM).
Bromodeoxyuridine incorporation studies showed that early keratinocyte growth was independent of mesenchymal support, but by 14 d, both fibroblasts and assembled basement membrane were required to sustain growth.
Thymidine incorporation studies showed LA inhibited T cell proliferation by 90%.
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The incorporation study was done by varying the concentration of one species while other two are kept constant.
The p-value for the E16, BrdU/EdU incorporation study is 0.07 and the p-value for the E17, BrdU/EdU incorporation study is 0.13.
Thymidine incorporation (studied at day 5 of culture) and (PKH -26 dilution assays (studied at day 7 of culture) were PKH -26edilutioncribed [ 35, 36].
The effect of TGZ on cell proliferation was determined by DNA measurement in the cultures and 5-bromo-2'-deoxyuridine incorporation study.
In a recent O-tracer incorporation study we also found indication for oxygen transport through Pt thin film electrodes [29] and in the very recent work in Ref. [30] the authors also suggest an oxygen transport through polycrystalline Pt electrodes to explain their experimental results.
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