As smaller proportions of the stored Mo pool are enzymatically incorporated ( fenz), expression of the fractionation associated with the incorporation step increases, and as a consequence the cumulative fractionation is larger.
This is because if the stored Mo pool is quantitatively incorporated into enzymes, then no isotopic fractionation is expressed from the enzymatic incorporation step.
Therefore, the generation of electron hole in the oxygen incorporation step and its recombining with electron in the oxygen desorption step seems to enhance the participation of active lattice oxygen in carbon oxidation and improve the catalytic oxidation performance.
Separate labeling reactions were pooled after each respective Cy dye incorporation step and then again divided into aliquots to minimize inconsistencies in probe generation.
The final step of incorporation, step 4, may take days or weeks.
The pyroluminescence intensity for each bead-containing well was used to determine base calls for each nucleotide incorporation step.
The separate labelling reactions were pooled after each respective Cy dye incorporation step and then again divided into aliquots to minimize inconsistencies in probe generation.
Because the precatalytic conformational change associated with these domain motions was rapid (step 5 in Figure 6), a rearrangement of the active site residues was instead proposed to limit the rate of correct nucleotide incorporation (step 6 in Figure 6).
The catalytic efficiencies of each of these mutants were analyzed for the incorporation step on control and adducted DNAs with the lesions being in both the major and minor grooves (Tables 3 and 4).
One such study using DNA labeled with the fluorescent reporter 2-aminopurine (2-AP) suggests the existence of several rapid noncovalent steps between the initial binding of dNTP and the covalent incorporation step in the catalytic mechanism of Dbh, the Dpo4 homologue from Sulfolobus acidocaldarius.
