The results are expressed as counts per minute of H-thymidine incorporation and represent the mean of triplicate cultures.
In HuH7 cells, the BrdU incorporation rates (representing DNA synthesis) in cultures treated with 5-FU alone and IFN- α/5-FU were 0.649±0.052 and 0.312±0.004, respectively.
We studied rapidly growing caiman juveniles maintained under constant optimal conditions, so our measured rates of incorporation probably represent higher rates for this species than those seen in wild caimans enduring episodic nutritional constraints and growing more slowly (e.g. adults).
Accordingly, their incorporation into tissue constructs represents a promising vascularization strategy in tissue engineering.
Hence, methodological flexibility which allows for future incorporation of additional indicators represents an important further requirement to a sound GMP monitoring program.
This approach of complex nanoparticle incorporation into polymer/cement composites represents a promising example for combining transcript therapy with biomechanical engineering.
Moreover, in the present study, we evaluate only PI incorporation by OBs, which represents a useful quantification of necrotic and late apoptotic cells [ 56].
Indeed the widely distributed simple barnase-barstar gene pairs might represent an incipient TI gene order without notable polymorphism, whereas the barnase cassette within larger polymorphic systems represents its incorporation into the fully developed versions of these systems.
We conclude that the upper pilosebaceous unit contains a distinct population of cells marked by Lrig1, which, based on their expression profile and incorporation of nucleoside analogue, represents one of the most proliferative cell compartments in telogen back skin.
