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Pulse injection of D2 and 13CO allowed tracing of the deuterium and CO incorporation pathway into the aldehyde product.
This altered incorporation pathway can bypass Vif's blockage, and consequently restore A3G's antiviral activity.
In the case of R88-A3G, the incorporation pathway of the fusion protein is different from that of A3G.
The deconstruction of β-5 and α,β ether linkages suggests the potential incorporation pathway between hydroxycinnamate and lignin units in wheat straw.
Likewise, kinetic data for mismatched dNTP incorporations by many DNA polymerases have been reported (7− 13), yet commonly used kinetic approaches prevent characterization of individual steps in the mismatched dNTP incorporation pathway.
This lack of impact of HIRA is likely explained by redundancy originating either from another H3.3 incorporation pathway depending on Arabidopsis orthologs of DAXX or DEK that encode H3.3 chaperones in mammals (Sawatsubashi et al., 2010; Elsässer et al., 2012).
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However, it should be noted that there is a functional connection between the sulfur incorporation pathways of thiamine biosynthesis and thiouridine synthesis in RNA [ 88, 120].
Polymerase fidelity, at its most basic level, is determined by the free energy difference between the highest energy barriers along the correct and incorrect dNTP 1 incorporation pathways.
While matched nucleotide incorporation by DNA polymerase β (Pol β) has been well-studied, a true understanding of polymerase fidelity requires comparison of both matched and mismatched dNTP incorporation pathways.
Although there is a supersaturation in the catalyst particle, the material incorporation pathways for sidewall growth differs from Au-induced growth, and it is possible that the material reaching the {111}B-type facets is limited due to a more favorable incorporation at the {111}A-type facets.
Linking our data to PRIDE (16), UniProt (17) and InterPro (18) will allow COSMIC somatic mutation data to be linked to protein and peptide annotation, while the addition of the Reactome (19) database will allow the incorporation of pathway data.
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