Suggestions(3)
Exact(5)
Metabolic labelling with L-[methyl-H]methionine in the presence of cycloheximide and chloramphenicol revealed tracer incorporation only in the long C/EBPβ isoforms (data not shown) (Pless et al, 2008).
This has been additionally demonstrated in the current study, where both BV VLPs and PB VLPs are "coated" in host cell outer membrane, but gp64 would be available for incorporation only into the BV VLPs.
The αERKO mouse has allowed us to show that estrogen-like effects of EGF require the ERα in vivo, as EGF induced PR mRNA and thymidine incorporation only in uteri from wild-type mice, indicating that the αERKO mouse was totally unresponsive.
We checked whether this assay compared well with the viability results obtained with the classical MTT test: the results were almost superimposable for ZD1839; it was not the case for C225, which exhibited acceptable dose effect curves with the thymidine incorporation only.
Subsequent studies, in which substrates specifically labeled with deuterium at C6 or C8 were used, showed that deuterium incorporation only at C8 results in significant suppression of lipoyl product formation and that sulfur insertion at C6 always precedes insertion at C8, even with an unlabeled substrate.
Similar(55)
It is presumed that the assimilation pattern was different between oligosaccharides (Additional file 1: Fig. S1) and polysaccharides (Fig. 4b), because not only incorporation but also degradation is important for the assimilation ratio.
One explanation for the complex mutation data in COS-7 and 293T cells is TLS by a highly error-prone DNA polymerase that made errors in nucleotide incorporation not only opposite the lesion but also opposite natural DNA bases near the cross-link site.
Longer incubation time results also in more D-loop extension, but 2D gel analysis revealed incorporation of only limited numbers of nucleotides (<100 nt) by either Pol η or Pol κ (Supplementary Fig. 3B D).
Unable to buy out its partner, it is pursuing local incorporation, something only recently allowed, and investments in Bank of Shanghai and Ping An, an insurance firm.
Incorporation of only 3 wt.
Neither incorporation of only one nor implementation of three interim analyses led to early stopping due to sufficient agreement.
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