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We investigated collagen accumulation by H-thymidine incorporation, MMP expression by substrate gel zymography and TIMP expression by Western blot analysis.
GMA MP incorporation increased expression of EMT markers and enhanced mesenchymal morphogenesis of PSC aggregates.
Developing de novo designs that can support cofactor incorporation upon expression in a cell is needed to support a synthetic biology advance that integrates with natural bioenergetic pathways.
Successful incorporation and expression of both genes using a linear purified DNA fragment, containing exclusively rearranged lciA, mutated pds and nabI* genes of H. pluvialis, represents to our knowledge the first functional self-cloning approach in microalgae, a breakthrough that can facilitate the widespread use of genetically altered microalgae.
Other organisms use related mechanisms of pseudogene incorporation into expression sites [16].
Cells were harvested and analyzed for BrdU incorporation and expression of phosphohistone H3.
Incorporation of expression status and promoter DMR status was subsequently undertaken.
Transformed lines were screened for transgene incorporation and expression using PCR and RT-PCR, respectively.
Future work will include incorporation of expression data to create directed edges showing the information flow between edges.
Proliferating cells were identified through BrdU incorporation and expression of the proliferation markers Ki67 and Cyclin D1.
As was done previously, selected codons in the Protein Z sequence were mutated to Amber codons (TAG) for BPA incorporation during expression.
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