Recently, the biosynthetic pathway of nectrisine in T. discophora was studied by conducting incorporation experiments using 13C-labeled substrates (Miyauchi et al. 2015).
We showed that the biosynthetic pathway of PP-V coincided with major Monascus pigments based on labeled acetate incorporation experiments (Ogihara et al. 2000a, b).
Biosyntheses of natural products were classically studied applying in vivo experiments, usually by combining incorporation experiments with stable-isotope labeled precursors with cross-feeding experiments of putative intermediates.
Advanced nuclear magnetic resonance (NMR) and mass spectrometry methods enable the quantitative assessment of all major isotopologue species present in metabolites recovered from isotope incorporation experiments.
Deuterium incorporation experiments were performed, in order to investigate the mechanism of the bioreduction: the formation of the corresponding substituted 2-benzylacrylaldehydes, as intermediates to be effectively reduced by Baker's Yeast, was suggested.
BrdU incorporation experiments confirmed these results.
BrdU incorporation experiments were performed on cells transiently transfected for 72 h with siRNA duplexes.
Ki67 staining and BrdU incorporation experiments indicated that a subpopulation of human NS cells may withdraw from the cell cycle.
However, multiple in vivo BrdU incorporation experiments failed to reveal an increase in proliferation of Id3−/− γδ T cells (Fig. S5) [35].
To this end we performed methionine/cystein S35 incorporation experiments followed by a western blot in both HeLa and SY5Y cells (fig. 5C and fig. S1D).
To determine whether the increase of cell number was caused by the promotion of cell cycle progression, we performed BrdU incorporation experiments.
