Exact(4)
However, the incorporation efficiency is generally low in mammalian cells.
For purine dNTPs, the incorporation efficiency is highest when dC/dG is the pBP and lowest when dA/dT is the pBP.
Although unAAs are typically incorporated into proteins in response to nonsense codon with good efficiency and excellent fidelity, further system optimization to increase the incorporation efficiency is still highly desirable.
In addition, PAR-CLIP is particularly suitable for studies using cultured cells, because of its high uptake efficiency of nucleoside analogues (incorporation efficiency is up to 4% for 4-SU in relative to uridine [18]).
Similar(56)
Fluorescence imaging of an SDS PAGE gel showed that all the amino acids are incorporated in response to the four-base codon; however, the incorporation efficiency was dependent on the structure of the side chains.
The incorporation efficiency was 60 85%.
Neither Env processing nor incorporation efficiency was predictive of viral transmissibility.
Dye incorporation efficiency was measured by NanoDrop Spectrophotometer.
Equilibrium dialysis indicated the ICG incorporation efficiency was 97.8 ± 0.6%.
Drug incorporation efficiency was expressed as in (2).
Dye incorporation efficiency was determined by using a Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions.
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