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Notably, p53 silencing led to comparable increases in BrdU incorporation compared with IGF-1 treatment alone, while additional IGF-1 treatment did not further increase BrdU incorporation in p53-ablated cells (Fig. 2B).
Cells stably overexpressing pBABE-G9a showed increased BrdU incorporation compared to control cells transfected with scrsiRNA (Fig. 5a c), whereas proliferation of cells expressing pBABE-G9a4KR was similar to siG9a cells.
The strain hardening index was calculated from stress–Henky strain data which obtained directly from biaxial measurement and the value increased with BGC incorporation compared to wheat flour dough.
Similarly, holes filling and closing method gives more control over the particle incorporation compared with that of groove filling and holes filling methods but requires more operations.
Recombinant CCN2/CTGF induced a 1.6 fold BrdU incorporation compared to unstimulated controls.
DKO lung EC also showed ∼4-fold decrease in BrDU incorporation compared to wild-type controls (Figure S3B).
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The results are given as BrdU-incorporation compared to untreated cells.
To examine whether Met135 contributes to the rotation of 8-oxoG into the syn conformation and thereby for Polκ's preference for A incorporation, we compared the catalytic efficiency of nucleotide incorporation opposite a non-damaged G and 8-oxoG by the wildtype Polκ and the mutant Polκ protein harboring a mutation of Met135 to alanine (M135A).
Surprisingly, the SBP2L-SBP2 chimera was also unable to promote Sec incorporation while the SBP2-SBP2L chimeretainedned 20% Sec incorporation activity compared to CT-SBP2.
Fatty acid treatment alone did not affect incorporation rates, and the combined IL-1α plus fatty acid treatment had no effect on [H]proline incorporation rates and a slight positive effect on [S]sulfate incorporation rates compared with IL-1α treatment alone.
To confirm the preferred position independently of CIZ1, we monitored the location of EdU-labelled Xis at the time of Xi replication/EdU incorporation (pulse) compared to its location 30 min later (chase), and relative to the nucleolar marker fibrillarin (Supplementary Fig. 1).
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